动物造模,血激素,Hsp⁃70 z-bo 2022-02-05 424

　　Objective: To explore the effects of noise pollution on the blood hormone levels and heat shock protein-70 (Hsp-70) expression in rats.

　　Method: 40 male Wistar rats were randomly divided into control group (normally raised) and experimental group (divided into three groups of 35, 65, 85dB), each with 10 animals. Stimulate once a day, each stimulation for 30 minutes, continuous Stimulate for 20 days. On the 21st day, blood was collected and the levels of norepinephrine (NA), testosterone (T), dopamine (DA) and Hsp-70 in the blood were detected by enzyme-linked immunosorbent assay (ELISA).

　　Results: ELISA method found that the weight of the experimental group (35, 65, 85dB group) rats were 23.45%, 30.13%, 35.64% less than the control group, respectively, T and DA levels were reduced by 9.12%, 20.06%, 37.99% And 15.49%, 18.31%, 24.88%; blood NA and Hsp-70 levels increased by 35.08%, 171.52%, 197.86% and 39.34%, 195.09%, 285.25%, respectively, the differences were significant (all P<0.01) ｡

　　Conclusion: Noise pollution can significantly affect the blood hormone levels and the expression of heat shock protein-70 in rats.