动物造模,心房钠尿肽,黑色素瘤 z-bo 2022-03-23 427

　　Objective: How to study the effect of atrial natriuretic peptide on the growth of melanoma?

　　Methods: The melanoma B16F10 cells cultured in vitro were subcutaneously inoculated into ANP knockout mice and C57BL/6J mice to establish melanin. The tumor volume was measured and calculated; 12 days after tumor inoculation, the mice were sacrificed, and the transplanted tumor was isolated and weighed. After embedding tumor tissue in paraffin, do you want to use immunohistochemistry to detect tumor cell proliferation and microvessel density?

　　Result: Is the tumor volume of ANP knockout mice significantly smaller than C57BL/6J mice, and the tumor weight is also significantly reduced? Are the cell proliferation and microvessel density in tumor tissues of ANP knockout mice significantly lower than those of C57BL/6J mice?

　　Conclusion: Can ANP deletion significantly inhibit the growth of melanoma by inhibiting tumor cell proliferation and microangiogenesis?