动物造模,姜黄素 z-bo 2020-09-08 383

　　OBJECTIVE: To investigate the content or activity changes of nuclear factor E2 transcription-related factor 2 (Nrf2) and anti-oxidative stress-related factors in rats with brain trauma, and to study the protective effect of curcumin on brain injury and anti-oxidative stress in rats.

　　Method: Did you choose 20 SPF male SD rats and divide them into normal control group? Brain injury model group (TBI group)? Brain injury solvent group (TBI + S group)? Brain injury curcumin treatment group (TBI) + C group), 5 in each group? Among them, the normal control group only received anesthesia and saline, while the TBI group? The TBI + S and TBI + C groups were modeled with a free fall craniocerebral injury model device, and then were given equal volumes of saline, DMSO carrier (0.05%) and curcumin (5 mg/kg). One day later, all rats were sacrificed and brain tissues were collected for RNA and protein extraction. Quantitative reverse transcription polymerase chain reaction (qRT? PCR) To detect Nrf2 mRNA expression, please use WesternBlot to detect Nrf2 protein expression? Chemical colorimetry to detect the content of malondialdehyde (MDA) and reduced glutathione (GSH) in rat brain tissue homogenate? Can the activity of catalase (CAT) and superoxide dismutase (SOD) be detected by enzyme-linked immunosorbent assay (ELISA) for inducible nitric oxide synthase (iNOS) and heme oxygenase? Content 1 (HO? 1)?

　　Result: Compared with the normal control group, the expression of Nrf2 mRNA and protein in the brain tissue of the TBI group? TBI+S Group has grown significantly, MDA content, iNOS and HO? 1 Increased vitality, glutathione content? Compared with the SOD and CAT TBI and TBI + S groups, the Nrf2 mRNA and protein expression levels in the TBI + C group were significantly reduced, and the MDA content was iNOS and HO1 activity. Both TBI group and TBI + S group decreased, GSH content, SOD and CAT activity increased, the difference was statistically significant (P\u003c0.05), and the difference was statistically significant (P\u003c0.05). )?

　　Conclusion: Curcumin has an anti-oxidative stress effect on rats with brain injury. Is it possible to reduce the expression of Nrf2 and change the relevant indicators of human body anti-oxidative damage that may have a protective effect on TBI brain tissue?