动物造模,二烯丙基二硫 z-bo 2022-02-05 364

　　Objective How to study the radioprotective effect of diallyl disulfide (DADS) on mouse spermatogenic cells?

　　Using Method 4Gy dose X-rays to establish animal radiation hazard models? Observe the changes in the testis tissue of irradiated mice? Sperm movement and abnormal sperm rate, protein carbonyl content, malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) content detection, radiation damage to spermatogenic cells and evaluation of spermatogenic ability DADS on cells Protect the tissue antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH? Px) activity and glutathione (GSH) content, by Western blot To detect the expression of Nrf2 signaling protein, DADS seeks Japanese radiation protection mechanism? Results Compared with the irradiation group alone, the DADS pretreatment group had less testicular tissue damage, significantly improved sperm motility (P \u003cu\→ u003c0.05), and reduced sperm deformities (P \u003cu\→ u0.05c0.05) . \u003c0.05). Protein carbonyl content? MDA and 8? OHdG level decreased significantly (P\u003c0.05)? turf? Cat? GSH? Is there a significant increase in Px, GSH and other antioxidant indicators (P\u003c0.05), and is the expression of Nrf2 significantly increased?