染色体畸变 z-bo 2020-09-08 406

　　Purpose: Do you want to investigate the influencing factors of the in vitro chromosome aberration test and the quality control of the chromosome preparation process, and jointly analyze the methods and key points of successful in vitro chromosome preparation?

　　Method: After using conventional hamster hypotonic, use Chinese hamster lung cells (CHL), cell culture and chromosome preparation, and select positive mutagenic mitomycin and cyclophosphamide as drugs. fixed? Put down the film and finally perform a microscopic examination on the film?

　　Result: CHL chromosome abnormality check, mitomycin? After cyclophosphamide treatment, the rate of chromosomal aberrations increased significantly, both were 20%. Under the two test conditions with and without metabolic activation system, is the rate of chromosome structure abnormalities in the negative control group less than 5%? Are the prepared chromosomes well dispersed, medium length, and high success rate? Conclusion: In the in vitro chromosome aberration test, there are many factors that affect the preparation of cell chromosome preparations. Every step is important. You need to closely follow the steps and learn the principles of each step. Can a good sample be prepared only through careful, patient and scientific operation?