动物造模,癫痫,甘草酸 z-bo 2020-09-08 354

　　Objective: To investigate the protective effect and mechanism of glycyrrhizic acid on hippocampal neuron damage in epileptic rats.

　　Method: Lithium chloride? Picopine was used to ignite a rat model of epilepsy. After successfully establishing the model, the rats were randomly divided into epilepsy group and glycyrrhizinate group, and untreated rats were used as normal control group. There are 12 people in each group. The issl and Tunel methods are used to detect the damage and apoptosis of rat hippocampal neurons, the JC-1 method is used to detect the mitochondrial membrane potential of rat hippocampal neurons, and the colorimetric method is used to detect aspartic acid proteolysis. Western blotting was used to detect the level of enzyme 3 (caspase3) and caspase-9 activity; caspase 3 and 9, B lymphoma 2 (Bcl 2), Bcl 2 related X protein (Bax), cytochrome we detected To C (CytC) and rat hippocampal tissue (Apaf? 1) protein expression apoptotic protease activator.

　　Results: Compared with the normal control group, the number of neurons in the epilepsy group decreased (P \u003cu003c0.01), the number of TUNEL positive cells increased (P \u003cu003c0.01), and the mitochondrial membrane potential caspase 3 and 9 The activity of (P\u003c0.01) decreases (P\u003c0.01), Bcl? 2 and CytC down-regulated mitochondrial expression (P\u003c0.01), and CytC and Apaf? Expression in Bax and cytoplasm. The expression of 1 is up-regulated (P\u003c0.01). ). Compared with the epilepsy group, the number of neuronal cells in the glycyrrhizic acid group increased (P≥0.01), the number of TUNEL positive cells decreased (P≥0.01), and the mitochondrial membrane potential increased (P≥0.01). \u003c0.01), decreased activity of caspase 3 and 9 (P\u003c0.01), up-regulation of CytC expression in Bcl-2 and mitochondria (P\u003c0.01), and expression of Bax and CytC in the cytoplasm. Apaf-1 expression is down-regulated (P\u003c0.01).

　　Conclusion: Glycyrrhizin can inhibit the damage of hippocampal neurons in epileptic rats by blocking the mitochondrial pathway.