动物造模 z-bo 2020-09-08 374

　　OBJECTIVE: To study the effect of Xiaoaiping injection on the pathological damage of liver tissue in rats with liver cancer induced by intraperitoneal injection of diethylnitrosamine, as well as its effect on liver cancer cell migration in hypoxic tumor microenvironment and its mechanism.

　　Method: Select 60 SD rats and divide them into 48 model groups and 12 normal control groups randomly. The model group was intraperitoneally injected with diethylnitrosamine to establish a liver cancer model, and the normal control group was intraperitoneally injected with the same amount of normal saline. After 14 weeks, the model group was randomly divided into 3 groups. The two groups were injected intraperitoneally with small and high doses of Xiaoaiping injection, and one group and the normal control group were injected with the same dose of normal saline 5 days a week for 4 weeks. Sampling was taken at the 19th week to detect the pathological changes of rat liver tissue. What is the effect of Xiaoaiping injection on SMMC of hepatocellular carcinoma cells under CoCl2-induced hypoxia? The effect of 7721 and HepG2 on migration was observed in the scratch healing experiment. At the same time, Xiao Aiping showed IL? The effect on 6mRNA expression was tested.

　　Result: Compared with the normal control group, the liver tissue of the model group showed obvious liver cancer-like changes, indicating that the liver cancer model can replicate normally. After treatment, compared with the model group, low-dose and high-dose Xiaoaiping reduced pathological damage to rat liver tissue; Xiaoaiping injection significantly inhibited HepG2 cell migration and reduced hypoxia. Inhibition of IL-6 mRNA expression in the environment At the same concentration, it can only inhibit the migration of SMMC 7721 cells and tend to inhibit the expression of IL6 mRNA, but the difference is not statistically significant. No one.

　　Conclusion: Can Xiaoaiping injection reduce the pathological damage to the liver tissue of primary liver cancer model rats by intermittent intraperitoneal injection of diethylnitrosamine and SMMC under chemically induced hypoxia? It may affect 7721 and HepG2 and have different inhibitory effects on cell migration. Down-regulating the expression of IL-6 mRNA may be one of the mechanisms that inhibit the migration of liver cancer cells.