动物造模,ＬＰＳ,脂多糖,莲心碱 z-bo 2020-09-08 373

　　Purpose: To investigate the effect of liensinine on acute lung injury (ALI) in mice induced by lipopolysaccharide (lipopolysaccharide, LPS).

　　Methods: BALB/c mice were randomly divided into control group, LPS group, LPS + liensinine (2, 4, 8 mg/kg) group, dexamethasone (5 mg/kg) group, and nasal instillation method was used to establish LPS induction Model of acute lung injury. Twelve hours later, histological observation of pathological changes in lung tissue; ELISA to detect the contents of TNF-α, IL-6 and IL-1β in alveolar lavage fluid (BALF); Wright-Giemsa staining to detect the number of neutrophils in BALF ; BCA method to detect total protein content; Evans Blue to detect lung capillary permeability; spectrophotometric method to detect MPO activity, MDA content, SOD activity, and GSH content in lung homogenate supernatant; flow cytometry to detect lung The ROS content in the organization.

　　Results: In the LPS group, inflammatory cell infiltration, bronchoalveolar wall thickening, and pulmonary congestion were seen in the lung tissues, while the liensinine group could improve lung injury; the contents of TNF-α, IL-6, and IL-1β in the BALF of the LPS group Significant increase, the number of neutrophils and total protein increased significantly, pulmonary capillary permeability, MPO activity and MDA content increased, SOD activity and GSH content decreased, and ROS content increased, while the liensinine group could reduce TNF in BALF- The content of α, IL-6, and IL-1β reduces the number of neutrophils and total protein content, reduces the permeability of lung capillaries, reduces MPO activity and MDA content, increases SOD activity and GSH content, and reduces ROS content.

　　Conclusion: Liensinine can protect LPS-induced acute lung injury in mice through anti-inflammatory and antioxidant effects.