动物造模,异氟烷麻醉 z-bo 2020-09-08 432

　　Purpose: To observe the changes in heart rate variability of rats under restraint fixation and isoflurane anesthesia (flow rate: 0.8 L, concentration: 1.5%), and the effects of restraint intervention and isoflurane anesthesia on rats for 30 days a day for a total of For 9 days to evaluate the effects of long-term isoflurane anesthesia and restraint fixation on the stress level of rats, the effects of hormones related to body weight, pain threshold and HPA axis were compared. By choosing an appropriate fixation method according to the rat, we provide an experimental method for basic medicine. Important reference?

　　Method: SD rats were randomly divided into 3 groups: blank group, restraint group, isoflurane anesthesia group? Acute experiments were performed by recording the rat’s ECG for 15 minutes to observe the heart rate changes and heart rate variability of the rats under restraint and isoflurane anesthesia? In a chronic study, the weight changes, pain threshold changes, and stress-related hormone levels of the three groups of rats before and after continuous treatment (30 minutes/day) were compared.

　　Results: 1) Acute experiment: Both restraint intervention and isoflurane anesthesia significantly increased the heart rate of rats. Compared with the blank group, is it statistically significant (P0.05)?

　　Conclusion: The above results indicate that low concentrations of isoflurane can cause anesthesia. In rats, changes in autonomic nerve function will lead to increased heart rate, decreased heart rate variability and mainly sympathetic excitement? Repeated restraint and fixation can easily activate the HPA axis of rats and cause chronic stress? Animals should be fixed for a long time. So, is isoflurane anesthesia better than restraint fixation?