动物造模,放射性肺损伤 z-bo 2022-02-04 403

　　Objective: To explore the protective effect of mild hypothermia on radiation-induced lung injury and compare it with the commonly used anti-radioactive drug Amifostine?

　　Method: 75 male SD rats were randomly divided into empty control group, simple irradiation group and ammonia water. Except for the blank control group, the phosphatase positive control group, the mild hypothermia prevention group, and the mild hypothermia treatment group received a 20 Gy electron beam full chest irradiation except for the blank control group, while the amifostine positive group received a 20 Gy electron beam before irradiation Receive amifostine intraperitoneally for 30 minutes. I received an internal injection. After the body temperature dropped to mild hypothermia, the rats in the hypothermia group were irradiated and kept for 6 hours. Whether to radiate the rats in the mild hypothermia treatment group to keep the body temperature at a low temperature for 6 hours? Pulmonary histopathological examination and collagen fiber examination were performed on 14th, 14th and 35th day after irradiation, and analysis of malondialdehyde (MDA), reduced glutathione (GSH) and superglutamine in rat serum . The content of oxidative dismutase activity (SOD) was determined. TNF homogenate in the tissue? Alpha content and lung tissue apoptosis?

　　Result: The SOD activity of each irradiation group decreased immediately after irradiation, and the SOD activity of the mild hypothermia group was higher than that of the irradiation group on the 14th day (P \u003c0.05); compared with the simple irradiation group. , On the 14th day after irradiation, the serum MDA content of the amifostine positive group and the mild hypothermia treatment group were significantly reduced (P\u003c0.05); the TNFα content of the irradiation group was significantly increased 6 hours after irradiation. , And the increase in the phosphorus-positive group and the mild hypothermia treatment group was lower than that of the ammonia simple irradiation group (P\u003c0.05); the apoptotic cells in the simple irradiation group increased, and they were widely distributed on the day of irradiation. Then, each intervention group is reduced accordingly, but isn't the group's error obvious?

　　Conclusion: Can mild hypothermia treatment improve the antioxidant capacity of radiation-induced lung injury rats? Anti-inflammatory and anti-apoptotic capabilities and the role of commonly used clinical drugs Amifostine?