动物造模,铁皮石斛多糖,慢性萎缩性胃炎 z-bo 2020-09-08 565

　　Purpose: Mouse N? methyl? '? Is it possible to study the effect of polysaccharides from Shirob on nitrosoguanidine (MNNG)-induced chronic atrophic gastritis (CAG) and its molecular mechanism?

　　Method: randomly select 120 rats, normal group, model group, Janweisch group, high-density officinol medicinal polysaccharide? in? Low-dose group, 20 per group? The model group, the Jan Vish group, the stone end medicinal material has high polysaccharides? in? The low-dose group was given 167μg/mL MNNG drinking solution, while the normal group was free to enjoy distilled water for 8 weeks. After the model was successfully established, 1.34 g/kg off polysaccharide extract crude drug was administered to the high-dose Off Polysaccharide Polysaccharide daily. The medium dose group is 0.67g/kg; the low dose group is 0.335g/kg? Does Yangwei Shuqun take 4.0g/kg Yangweisu granules every day? Is the model group fed with saline, but the normal group has no intervention? Will you take out the stomach tissue for pathological examination after 12 weeks? Gastrin (gas)? Interleukin 6 (IL? 6)? Proliferating cell nuclear antigen (PCNA)? Tumor necrosis factor? α (Tumor Necrosis Factor α, TNFααmRNA)? Janus Kinase 2 (JAK2)? Activator? 3/Detected tyrosine phosphorylation 3 (p?STAT3/STAT3)? Results After 12 weeks of treatment, is the polysaccharide content of stone D in the normal group high? Weight of the low-dose group? The Yang Weishu group was higher than the model group (P \u003cu\→ u003c0.05); the body weight of the high-dose officin C-polysaccharide group was higher than that of the Yang Weishu group (P\u003c0.05); ②The inflammation in the model group was higher In the normal group (P\u003c0.01), the number of glands, the thickness of the glands, the number of principal cells and the number of parietal cells were all lower than the model group (P\u003c0.05). What is the grade of the high, medium, and low-dose group and the stomach nourishing group? How many glands? Gland thickness? Main cell number? Compared with the model group, the number of parietal cells recovered (P\u003c0.05)? Compared with the foreign flavoring group, the high polysaccharide of officinale was higher, and the number of main cells in the medium-dose group increased ((P\u003c0.05); the number of parietal cells in the high-dose offsite polysaccharide group increased. (P\u003c0.05) ); ③The serum GAS levels of the normal group, high, medium and low dose Shiden polysaccharide group and Jan Weish group were higher than the model group (P\u003c0.05); (P\u003c0.05)? Normal group high-dose iron skin Serum IL-6 levels in the polysaccharide group were lower than the model group (P\u003c0.05). Model group (P\u003c0.05); ⑤normal group, high-dose group of end medicinal polysaccharide; JAK2?p?STAT3/ The expression of STAT3 protein is higher than that of the model group. Conclusion Shi D medicinal polysaccharide has a good protective and reversal effect on CAG rats, which can effectively restore, reduce weight, and reduce gastric mucosal atrophy. The mechanism may be through inhibition of JAK2/p?STAT3 signal Activation of pathways to improve gastric mucosal pathology.