动物造模,杜仲提取液,乳腺炎 z-bo 2022-02-19 385

　　Objective: How to study the therapeutic effect and behavior of Eucommia ulmoides extract on mice with mastitis?

　　Method: A mouse mastitis model was established by injecting LPS into the mammary duct, and 12 hours after LPS injection, different concentrations of Eucommia ulmoides were administered intraperitoneally and divided into 6 parts. group? Twelve hours after the administration, the mice were dissected, breast tissues were collected, the pathological changes of each group were observed by HE staining, and the cytokine changes of TNFα2αβILβ6βILβ1 in the breast tissue were detected by ELISA.

　　Results: 1) LPS concentration of 50μL 0.2 mg/mL can successfully establish a mouse mastitis model. (2) HE staining results show that Eucommia ulmoides extract can alleviate LPS-induced mastitis in mice. The increase in the dosage of Eucommia ulmoides extract reduces the damage of breast tissue, and the injection doses of 20 mg/kg and 27 mg/kg respectively have the best effect; (3) ELISA results show that Eucommia ulmoides extract can reduce inflammatory cytokines in breast tissue IL? 1β? IL? 6. Does tumor necrosis factor have the highest effect when the alpha content and injection volume are 27 mg/kg (P\u003c0.01)?

　　Conclusion: Can the extract of Eucommia ulmoides significantly reduce the pathological damage to the breast tissue of mice? Release of internal inflammatory cytokines that reduce breast tissue can prevent the recruitment and migration of neutrophils into breast tissue and acinar cavity, thereby reducing the damage to breast tissue at a concentration of 27 mg/ml. Does one kilogram of Eucommia extract have The best anti-inflammatory effect?