动物造模,大黄素 z-bo 2020-09-09 387

　　Objective: To observe the effect of emodin on fat browning in apolipoprotein knockout (ApoE-/-) mice, and to explore the mechanism of improving hyperlipidemia and other congestion?

　　Method: After 12 weeks of high-fat diet, 8-week-old male ApoE-/- mice were randomly divided into 3 groups. Model group? Simvastatin 5.7 mg/kg group? Emodin 80 mg/kg group; male C57BL/6J mice of the same age served as normal control group and received basic diet. Each group of mice took corresponding drugs or drinking water for 18 weeks according to the dose. Test indicators include body weight (BW) and the weight of inguinal white adipose tissue (inguinal white adipose tissue, iWAT)? The weight of brown adipose tissue (BAT)? Blood lipids? Cardiac Function? What are the pathological features of iWAT and uncoupling protein 1 (Uncoupling protein UCP1) iWAT?

　　Result: Emodin can significantly reduce the weight of mice (P\u003c 0.05)? Are iWAT weight/weight ratio (P\u003c0.05) and serum TCγTG content (P\u003c0.05), BAT weight/weight ratio (P\u003c0.05) and cardiac ejection fraction (ejection fraction, EF) increased? Short axis shortening rate (score shortening, FS) (P\u003c0.01); HE staining results show that iWAT cells seem to be multi-compartmental, the cells become smaller and rounder, and the tissue becomes denser. Immunohistochemistry results show that, The positive expression of UCP1 protein in iWAT is average. Does the optical density (average optical density, AOD) increase significantly (P\u003c0.01)?

　　Conclusion: Emodin promotes the browning of white adipose tissue in the groin of ApoE-/- mice, reduces the accumulation of white fat, and is believed to be related to the alleviation of hypertension. Improve symptoms. What is your role?