动物造模,记忆损害 z-bo 2020-09-09 373

　　Objective: To study the effect and mechanism of Xingnao Zaizao Capsules on memory impairment in mice induced by formaldehyde?

　　Method: SPF grade KM mice were randomly divided into empty control group, solvent control group and low, medium and high dose groups. Are there 5 groups with 14 mice in each group and 4 groups except the blank control group exposed to the formaldehyde poisoning device for 14 days? The learning and memory abilities of each group of mice were tested in a dark experiment; the levels of nitric oxide synthase (NOS), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the brain tissue were determined by biochemical methods Hematoxylin-eosin staining was used to observe the number of pyramidal cells in the CA1 region of the mouse hippocampus. What are the changes in form and structure?

　　Results: Compared with the blank control group, mice in the solvent control group had a shorter period of avoiding darkness (P\u003c0.01), the number of errors increased (P\u003c0.01), and the activity of NOS and SOD in brain tissue increased and decreased (P≥ 0.05), the level of MDA increased (P≥0.01), the number of pyramidal cells in hippocampal CA1 area decreased, the arrangement was sparse and disorderly, and the shape was irregular. Compared with the vehicle control group, the incubation period of mice in the medium and high dose groups was prolonged (P\u003c0.05), NOS and SOD activities were increased (P\u003c0.01), while the number of errors in the low, medium and high dose mice was reduced (P\u003c0.05), the level of MDA decreased (P\u003c0.01). The number of pyramidal cells in the CA1 region of the mouse hippocampus increased slightly. In the high-dose group, is the level of pyramidal cells in the CA1 region of the hippocampus obvious, tightly arranged and the number increased significantly?

　　Conclusion: Xingnao Zaizao Capsule can improve the memory impairment induced by formaldehyde in mice, because its mechanism is related to its anti-oxidative damage and the increase in the number of pyramidal cells in the hippocampal CA1 area.