动物造模,2型糖尿病,N-乙酰半胱氨酸 z-bo 2020-09-09 379

　　Objective: To explore the effect of antioxidant N-acetylcysteine (NAC) on liver oxidative stress and FoxO1 activity in type 2 diabetic rats?

　　Method: randomly divide 24 male SD rats into normal control group (C)? Diabetes non-treatment group (D) and NAC treatment group (D + AC) (n = 8)? Low-dose intraperitoneal injection of streptozotocin (STZ) and a high-fat diet model were used to establish type 2 diabetic rats. The D+AC group was given NAC 1.5 g/kg by gavage every day, and the C and D groups were given the same amount of normal saline. After 8 weeks, does the automatic biochemical analyzer detect plasma triglycerides (TG)? Free fatty acids (FFA)? Aspartate aminotransferase (AST)? Alanine aminotransferase (ALT) level, is the kit liver superoxide dismutase (SOD)? Catalase (CAT)? Glutathione peroxidase (GSH-Px) adenosine triphosphate (ATP) content and lipid peroxide malondialdehyde (MDA) content in plasma and liver tissue; Westernblot analyzed the expression level of caspase-3 in liver tissue and Analyze the expression level of FoxO1 protein in the nucleus. FoxO1 activity level?

　　Result: Compared with group C in plasma TG? FFA? AST? ALT? MDA and MDA content in liver tissue? Caspase 3 expression level? The FoxO1 activity of rats in group D was significantly increased, and the liver tissue SOD? Cat? ATP levels and GSH-Px activity are significantly reduced; can AC treatment inhibit the changes of the above indicators in diabetic rats after 8 weeks, is the difference statistically significant?

　　Conclusion: Antioxidant NAC can reduce diabetes-related liver dysfunction. This is the mechanism by which the level of oxidative stress in diabetes may be related to mitochondrial dysfunction and FoxO1 overactivation. Do you have any?