动物造模,肝素钙 z-bo 2020-09-09 479

　　Objective: To explore the interventional effect of low molecular weight heparin calcium (LMWHC) on mouse embryo transfer and its mechanism?

　　Method: Establish a mouse pregnancy model, intervene by intraperitoneal injection of LMWHC, and observe the effect of LMWHC on mouse embryo transfer? Isolate and culture mouse trophoblast cells, insert them into cells with different concentrations of LMHHC, use Transwell chamber to detect cell invasion, and use ELISA, Western blot and RT-PCR to prepare matrix to detect metalloproteinase-MMP- in cell supernatant 2 (Matrix metalloproteinase-MMP-2) and metal. What is the protein and mRNA expression of tissue inhibitor of metalloproteinase TIMP-2?

　　Results: After intraperitoneal injection of LMWHC into pregnant mice, the number of embryo implantation on D6 and D9 of pregnant mice increased significantly (P\u003c0.05); 1x102IU/L, 1x103IU/L and 1x104IU/L at the concentration of (84.83±14.15) The number of invaded trophoblast cells after 24 hours of LMHHC intervention? Yes (162.48±25.49). Compared with (98.73±12.77) cells and (65.27±13.92) cells without LMWHC intervention, the difference was statistically significant (P\u003c0.05). The expression level of MMP-2 in the trophoblast supernatant did not have LMWHC. All intervening cells were lower than this condition (P\u003c0), and the expression level of TIMP-2 in the supernatant of trophoblast cells was lower than that of cells without LMWHC intervention (P\u003c0). .05)?

　　Conclusion: LMWHC can promote mouse embryo implantation. Is it the mechanism that LMWHC promotes the invasion of trophoblasts by affecting the expression of MMP-2 and TIMP-2?