动物造模,姜黄素,慢性肾病,肾纤维化 z-bo 2020-09-10 375

　　Objective: To study the protective effect of curcumin on renal fibrosis in chronic kidney disease (CKD) and to explore the possible mechanism?

　　Method: 30 C57BL/6J mice were randomly divided into sham operation control group (NC) 5/6 kidney ligation. Model group (LIG) and curcumin treatment group (LIG + CUR), each group of 10 animals, CKD animal models were created according to the improved 5/6 kidney ligation method, and mice in the curcumin group received a curcumin-containing diet (100 mg ). /Day) (kg), the rest of the groups were fed a normal diet, and the mice were sacrificed after 3 months. The fibrosis index α-SMA and Hippo pathway transcription involved in the fibrosis activator of chronic kidney disease Yap? I tested the results of the kidney function test. Compared with the sham operation group, the ligation group has significantly increased urea nitrogen (BUN) and blood creatinine (Scr), and curcumin can effectively protect renal insufficiency; H&E Masson and immunohistochemistry results It was shown that the kidneys of mice in the ligation group had obvious kidneys. Despite renal tubular pathology and certain fibrotic changes, the degree of fibrosis was significantly reduced after curcumin administration. (P\u003c0.05), after modeling, the mRNA and protein levels of Yap protein increased, but the curcumin level decreased significantly (P\u003c0.05)?

　　Conclusion: Curcumin can effectively improve chronic kidney disease caused by 5/6 kidney ligation and reduce fibrotic lesions. Do the preliminary results of the mechanism study suggest that it may be related to the decreased Yap expression in the hippo signaling pathway?