动物造模,高血压 z-bo 2020-09-10 352

　　Objective: To study the effect of Astragalus on the endoplasmic reticulum stress response in vascular remodeling in hypertensive rats, and to explore the molecular mechanism of vascular protection?

　　Method: In the intervention group, 140 rats were divided into control group and model group, and abdominal aortic valve stenosis was used to establish a hypertensive rat model. The rats in the intervention group were intraperitoneally injected with Astragalus injection 8 g/(kg?D). Rat tails were used in each group 1, 2, 4, and 6 weeks after surgery. Can arterial pressure measurement measure blood pressure and vascular muscle thickness in rats? Can Western Blot measure CRT and Caspase-12 expression? Can the TUNEL method measure the apoptosis rate of vascular smooth muscle cells (VSMC)?

　　Result: The morphological changes of VSMC, blood pressure, arterial wall muscular layer thickness and VSMC apoptosis rate may increase over time in the model group. The expression of the ERS molecule CRT increased significantly 1 to 2 weeks after surgery, and decreased at 4 to 6 weeks, but after 2 weeks it was 12 caspase molecules. Expression increases and delays over time. Does this high expression make more sense? After enge intervention, the morphology of VSMC was improved to a certain extent compared with the model group. Blood pressure, blood vessel wall muscle layer thickness and VSMC apoptosis rate were significantly reduced. At the same time, Renge was CRT at 6 weeks. May inhibit the early high expression and high expression of caspase 12. Will this inhibition become more and more obvious over time?

　　Conclusion: Renge has a specific hypotensive effect on hypertensive rats. Can this mechanism participate in ERS protection and regulation of pro-apoptotic factors?