动物造模,生理盐,肉鸡盲肠微生物,短链脂肪酸 z-bo 2022-10-10 423

　　Objective: To study the effect of physiological saline intervention on the intestinal flora and short-chain fatty acid content of broiler chickens, and to provide specific evidence for early microbial intervention experimental research?

　　Method: 80 primiparous women were selected and randomly divided into two groups: control group (group C) and normal saline group (group S). Two days after shooting, chicks in group S received 0.5 mL of sterile normal saline per day, while group C did not.

　　Treatment: On the 3rd and 7th days, 8 chickens in the two groups were randomly selected, weighed, and the cecal contents were killed. The cecum content was determined by IlluminaMiseq high-throughput sequencing technology, and gas chromatography was used. How to determine the content of short-chain fatty acids in the cecum?

　　Results: The experimental intervention of physiological saline did not significantly affect the average daily gain of broilers in the early stage (P \u003c0.05). At the door level, the proportions of the two groups of broilers were basically similar, with iron powder, Proteus and Bacteroides as the main bacteria in the intestines of early broilers. At the genus level, the cecum of group S broilers was 3 days old. The relative contents of Bacteroides and cyanobacteria were 160% and 143% higher than those of group C, respectively (P0.05)?

　　Conclusion: The experimental intervention of physiological saline has a certain effect on the structure of the caecal flora and the content of short-chain fatty acids in three-day-old broilers, but this effect is not continuous. Will it gradually disappear with age?