动物造模,高脂食物,ApoE基因敲除,免疫系统 z-bo 2020-09-10 554

　　Objective: To detect the effect of high-fat diet and K/BxN serum on the distribution of immune cells in ApoE knockout (ApoE-/-) mice

　　Method: ApoE-/-fed mice were 8-week-old high-fat diet. At 1 week-old, K/BxN serum containing 17 kinds of anti-glucose-6-phosphate glucose isomerase (GPI) antibodies was injected intraperitoneally once a week, control The group was given normal food. The method of each injection of K/BxN is the same, each injection of 02mL, the serum was tested before and after the K/BxN serum was injected by ELISA method to detect the changes in the blood lipid level of mice. Ogisu can measure the swelling of the ankle joint. High-fat food and K/BxN serum were treated to 26 weeks of age. After flow cytometry antibody staining, spleen cells and bone marrow cells were separated.

　　Results: Intraperitoneal injection of K/BxN serum low-density lipoprotein cholesterol (LDL-C) before and after the concentration of high-fat diet ApoE mice, total cholesterol (TCHO) and triglyceride (TG) levels increased significantly, aortic valve The area of the plaque at the bottom increased significantly, so whether the ApoE-/- mice were injected with K/BxN serum high-fat diet. It may cause the symptoms of arteriosclerosis, and it is more severe than ApoE-/- mice fed a normal diet. Before intraperitoneal injection of K/BxN serum, the ankle joints and clinical scores of each group of ApoE-/- mice were at normal levels. The ankle width and arthritis score of ApoE-/- mice that were injected intraperitoneally with K/BxN serum high-fat diet were lower than those of ApoE-/- mice on normal diet. Blood flow test showed that after high-fat diet. K/BxN serum was injected. ApoE-/- down-regulates CD3 + T and CD19 + B cells in mouse spleen and bone marrow, and up-regulates CD11b + macrophages

　　Conclusion: High-fat diet and K/BxN serum are ApoE-/- inducing the down-regulation of CD3 + T cells and CD19 + B cells in the spleen and bone marrow of mice, and the up-regulation of CD11b + macrophages leads to the symptoms of arteriosclerosis in ApoE-/- mice Exacerbate and reduce joint inflammation