动物造模,荷瘤裸鼠 z-bo 2020-09-10 351

　　Purpose: How to wrap Nile Red in nano-specimen and use its fluorescence characteristics to study the tumor targeting of tumor-bearing mice and the tissue distribution of nano-specimen?

　　Method: Does H1688 use the CCK-8 method to detect Nile Red nanoparticles from the cytotoxicity of milk [NRNE(O)]? A BALB/c nude mouse lung cancer model was established, Nileed Suspension (NRS) and NRNE (O) were administered in the stomach, and lung cancer was observed using a small animal in vivo imaging system. What is the dynamic distribution of fluorescence intensity in tumor nude mice?

　　Result: NRNE(O) has lower cytotoxicity than NRS, good biocompatibility, and higher absorption in the body and accumulation of tumor sites. Is NR fast and stable using nano formulations? Does it clearly reflect the dynamic distribution of nano-morphology in mice?

　　Conclusion: The NRNE(O) prepared in this study is highly stable and uses Nile Red as a tracer. Does the application of nano-formulations to animals provide an important research method?