动物实验,阻燃剂TCPP z-bo 2020-09-10 365

　　Objective: To study the neurotoxicity of the flame retardant TCPP (tris(2~chloroisopropyl) phosphate) on mice and its related mechanisms

　　Method: randomly select 30 adult KM mice (kg? D) in the normal control group (0 mg /)? The low-dose (TCPP) group (10 mg/(kg·D)) and the high-dose TCPP group (100 mg/(kg·D)) continued to be administered intragastrically for 30 days and were observed after the end of exposure. The weight and general condition water maze test is used to test the learning and memory abilities of mice to determine the total triiodotyrosine (TT3) and free triiodotyrosine (FT3) in the mouse serum. To this end, we used a chemiluminescence immunoassay. Determination of total tetraiodotyrosine (TT4) and free tetraiodotyrosine (FT4) by colorimetry of glutathione transferase (GST) and superoxide dismutase (SOD) in mouse brain tissue Level? Malon Dialdehyde (MDA)?

　　Result: Compared with the control group, the water consumption of the TCPP high-dose group mice was significantly reduced (P\u003c005). The liver and spleen organ indexes increased significantly. (P\u003c005) In the TCPP exposure group, the escape latency of the water maze experiment was longer than that of the normal group (P\u003c0.05), and the total swimming distance of mice in the high-dose group increased significantly (P\u003c0). .05). The residence time in the target quarter circle has also been greatly reduced (P\u003c0.05). The mice in the high-dose TCPP exposure group were compared with the control group TT3 and the mice in the high-dose TCPP exposure group were compared with the control group TT3. It shows a significant increase in FT3 (P\u003c0.05). sale tax? Compared with the control group, the SOD of the mice in the low-dose TCPP exposure group was significantly reduced, and the MDA was significantly increased (P\u003c0.05). Compared with the control group, GST only decreased and MDA increased.

　　Conclusion: TCPP exposure is obviously neurotoxic, which can cause learning and memory loss in mice. The toxicity mechanism may be related to the oxidative damage to the brain tissue and the increase of thyroid hormone.