动物造模,慢性阻塞性肺 z-bo 2020-09-10 368

　　Objective: To reduce the lung tissue damage of chronic obstructive pulmonary disease (chronic obstructive pulmonary disease, COPD) and recombinant rat CC16 (rCC16) on the lung tissue of the mouse model of matrix metalloproteinase (MMP)-9 and matrix metalloproteinase tissue . ) Regulate the expression of repressor (TIMP)-1 and study the role of proteins?

　　Method: 40 clean C57BL/6 mice were randomly divided into 4 groups, namely the blank group? COPD model group? Divided into rCC16 dose group 1 and dose group 2, except for the blank group, all other mice smoked for 3 months to prepare COPD models. From the third month, the blank group and the model group were given PBS nasal drops, and the rCC16 dose group 1 and dose group 2 were given 1 μg/g body weight and 2.5 μg respectively. /gCC16 Will nose weight reduce intervention? Fluorescence quantitative polymerase chain reaction, observe the changes in the mental state, diet, weight and urination of each group of mice by H&E staining, and observe the changes in the morphology and structure of the lung tissue of each group of mice? Histochemical detection of immune MMP-9 and TIMP-1 messenger RMA (mRNA) and changes in protein expression?

　　Result: The weight of mice in the blank group increased with the increase of the feeding cycle, while the weight of mice in the COPD group was significantly lower than that of the blank group. After the intervention, the weight of mice in intervention group 2 increased every week, while the weight of mice in intervention group 1 increased slowly, the difference was statistically significant (P\u003c0.05). The lung tissue structure of the mice in the COPD model group was significantly destroyed. After the alveolar interval rCC16 intervention group, the alveolar structure of the mice tended to be intact and the alveolar formation was also reduced; the expression of MMP-9 and TIMP-1 in the lung tissue of the COPD model group was significantly higher In the blank group (P\u003c0.05); CC16 may reduce the expression of MMP-9 and TIMP-1 after the intervention, the difference is statistically significant (P\u003c0.05), and rCC16 has a reduction effect on MMP-9 Is TIMP-1 not dose-dependently regulated?

　　Conclusion: RCC 16 nasal intervention reduced lung tissue damage in COPD mice, decreased the expression of MMP-9 and TIMP-1 in lung tissue, and affected COPD. Is the treatment active?