动物造模,缺氧缺血性脑病,脑水肿 z-bo 2020-09-10 353

　　Objective: To investigate the effect of edaraben on cerebral edema and CD163/HO-1 signaling pathway in neonatal rats with hypoxic-ischemic encephalopathy (HIE)?

　　Method: 120 SD rats for 7 days, 90 of them were randomly modeled using double ligation of the left common carotid artery and hypoxia to establish a hypoxic-ischemic brain injury (HIBD) model. Divided into edarabon group and edarabon group, only ligate the other 30 without ligation, and use hypoxia as a sham operation: for the edarabon group, use edarabon 2 mg/kg once a day immediately after modeling, and intraperitoneal injection for 5 consecutive days. The model group and the sham operation group were injected intraperitoneally with the same amount of normal saline at the same time. Carefully observe the biological behavior of SD newborn rats in each group. TTC staining can observe the brain tissue morphology of each group of SD newborn rats 24 hours after modeling. After modeling, each group was tested at 6, 12, 24 hours, 2, 3, and 5 days. Changes of brain water content in SD neonatal rats; CD163? Fluorescence quantitative PCR (qRT-PCR) technology detects HO-1 mRNA expression level; CD163? How to detect the expression level of HO-1 protein by Western blot?

　　Results: 90 SD neonatal rats showed symptoms of lethargy, lethargy and cramps after the operation. After treatment with edaravone, the symptoms were significantly reduced compared with the model group and improved within 3 days. Twenty-four hours after modeling, TTC staining of the left hemisphere of newborn rats in the model group showed edema and pale color. , Its volume is slightly larger than that of the right hemisphere, and the degree of brain damage is significantly reduced after edaravon treatment. In the model group, the infarct area of the left hemisphere of neonatal rats in the Idarabong group was significantly larger than that in the sham operation group (P\u003c0.05). The area of cerebral infarction after lavender treatment was significantly smaller than that of the model group (P\u003c0.05). The brain tissue water content test results showed that after 6 hours of modeling, the brain tissue water content of the model group and edaravone group was significantly higher than that of the sham operation group. In the group (P\u003c0.05), is the brain water content the highest within 2 days? After edaravone treatment, the brain tissue water content of newborn rats was significantly lower than that of the model group (P\u003c0.05). The results of qRT-PCR and Westernblot were compared with the sham operation group. In contrast, the expression levels of CD163 HO-1 mRNA and protein in the brain tissue of neonatal rats in the model group edaravone group were significantly increased (P\u003c0.05), and the neonatal rats were treated with edaravone The expression of CD163 HO-1 mRNA and CD163 HO-1 mRNA was significantly increased. Protein expression level Is the protein expression level significantly higher than the model group (P\u003c0.05)?

　　Conclusion: Edarabine can significantly reduce cerebral edema and cerebral infarction in HIBD neonatal rats. Could this mechanism be involved in the activation of the CD163/HO-1 signaling pathway?