动物造模,二甲双胍,2型糖尿病 z-bo 2023-01-18 562

　　Objective: How to evaluate the effects of aerobic exercise and metformin combined with aerobic exercise on vascular oxidative stress in type 2 diabetic rats?

　　Method: Adopt a high-fat diet and take a small amount of STZ to induce type 2 diabetes model. It is divided into 5 groups: normal control group (NC), diabetes control group (DC), diabetic exercise group (DS), diabetic metformin intervention group (DM), diabetic metformin + exercise combined intervention group (DMS) . After 8 weeks of intervention, are you using HE staining for pathology? Western blotting and ELISA methods to detect rat blood vessel morphology, tissue protein expression and serum indicators?

　　Result: DS? Are the fasting blood glucose and insulin reductions in the DM and DMS groups different? Compared with the DC group, DS? The expression of Nrf2 and HO-1 in the blood vessels of the DM and DMS groups increased, and the expression of NOX4 protein decreased. The activity of SOD in blood vessels increased in DS and DMS groups, while MDA in DM and DMS groups decreased. DS? Did the levels of serum VCAM-1 and TNF-α in DM and DMS groups decrease to different degrees?

　　Conclusion: Can metformin and aerobic exercise reduce the vascular oxidative stress in type 2 diabetic rats by regulating the balance between Nrf2 and NOX4?