动物造模,血清及肺组织氧化应激指标 z-bo 2022-02-19 401

　　Objective: To study the effects of methylene blue and sevoflurane on oxidative stress indicators and inflammatory factors in serum and lung tissue of rats with lung ischemia-reperfusion (IR) injury?

　　Methods: 60 male SD rats were randomly numbered, sham operation group (blank control group), lung ischemia reperfusion group (IR control group), methylene blue pretreatment group (MB group), sevoflurane pretreatment group (S). Group), 15 rats in each group? After establishing an isolated lung ischemia-reperfusion injury model, we will compare SD rats treated in various ways, and compare the effects of interleukin-1β (IL-1β) on serum and lung tissue. -6 (IL-6), tumor necrosis factor alpha (tumor necrosis, TNF-α) and other inflammatory indicators as well as superoxide dismutase (SOD), malondialdehyde (MDA), reactive oxygen species (ROS) and adenosine Triphosphate (ATP) and other indicators of oxidative stress, hematology, and the impact of lung dry and wet weight?

　　Result: What is the dry weight ratio of lung tissue in MB group? The S group was significantly higher than the sham operation group, and the IR group was significantly lower than the IR group; the SOD and ATP levels of the MB and S group sham operation groups were statistically significant (P0.05). MDA and ROS were significantly higher than the sham operation group, and significantly lower than the IR group, while the statistical difference (P0.05) was significantly lower than the IR group. MB group? The lung tissue and serum levels of each inflammation index in the S group were significantly higher than those in the sham operation group, while the IR group was significantly lower than the sham operation group. The difference was statistically significant (P0.05)? The erythrocyte deformability index, blood coagulation index and plasma viscosity in T1 and T2 phases of MB group S are lower than T0 phase, and T1 and T2 phase indexes of MB group are lower than those of S group. Is the difference statistically significant (P\u003c0.05) ?

　　Conclusion: Both methylene blue and sevoflurane can improve the oxidative stress index of lung tissue in rats with lung ischemia-reperfusion injury, reduce serum and lung tissue inflammatory factors, and improve edema, while methylene blue can improve blood edema. You can increase the viscosity. More importantly, can you effectively reduce the risk of thrombosis?