动物造模,急性脑梗死 z-bo 2022-02-22 429

　　Objective: To study the protective effect of Shenmai injection on the brain tissue of mice with acute cerebral infarction, and to preliminarily explore its possible mechanism.

　　Method: This experiment studied 30 adult male CDs. -1 mice were randomly divided into control group. The model group and the experimental group each have 10 animals. A mouse model of acute cerebral infarction was created by suture. The experimental group was treated with 0.3mL Shenmai injection, and the control group and model group were treated with the same normal saline. What are the red blood cell function indexes of each group of mice? Do you use the TUNEL method to detect the apoptosis rate of mouse cerebral cortex neurons and the expression of carpine-1 and Bcl-2 by Western blot and q-PCR?

　　Result: Compared with the control group, the RBC-C3bR of the experimental group decreased significantly, and the RBC-ICR increased significantly (P\u003c0.05)? Compared with the model group, the experimental group RBC-C3bR significantly increased, RBC-ICR significantly decreased (P\u003c0.05)? The number of TUNEL positive cells in the brain tissue slices of the experimental group of the model group was significantly higher than that of the control group (P\u003c0.05); significantly less than that of the model group (P\u003c0.05)? Compared with the control group, the carpine 1 protein and mRNA expression levels of the model group and the experimental group were significantly increased, and the Bcl-2 protein and mRNA expression levels were significantly reduced (P\u003c0). .05); Compared with mice in the model group, the Carpine 1 protein and mRNA expression levels of the experimental group mice were significantly reduced, and the Bcl-2 protein and mRNA expression levels were significantly increased (P\u003c0). .05)? The possible mechanism to inhibit neuronal apoptosis is to down-regulate the expression of carpine 1 protein and up-regulate the expression of anti-apoptotic protein Bcl-2, thereby exerting neuroprotective effects on brain tissue.