动物实验,博来霉素,肺纤维化 z-bo 2020-09-10 490

　　Purpose: Three different methods are used to inject bleomycin into the trachea to perform a lumbar puncture needle tracheal intubation model: lumbar puncture needle tracheal intubation, indwelling needle tracheal intubation and tracheotomy to induce lungs in C57BL/6J mice Fibrosis. explore? How do the three indwelling needle tracheal intubation models and tracheal incision models affect mice?

　　Method: 120 C57BL/6J mice were randomly divided into a blank group? Lumbar puncture needle tracheal intubation model group? In the indwelling needle tracheal intubation model group and the tracheotomy model group, did you use different methods to inject bleomycin into the trachea and observe the general condition of the mice (weight change and survival)? Histopathological changes (HE and Masson staining) and hydroxyproline levels?

　　Result: Lumbar puncture needle tracheal intubation model group? 28 days after modeling, in the indwelling needle tracheal intubation model group, the weight of the mice was heavier than the tracheostomy model group, which was statistically different. (P\u003c0.01?P\u003c0.001); There are differences in the survival rate of mice in each group. Except for the blank group, the tracheotomy model group was the lowest, and the indwelling needle tracheotomy model group was the highest. There were statistical differences between the blank group and the tracheotomy model, and there was no statistical difference between the other groups. (P\u003c0.05) Does the degree of alveolar inflammation and pulmonary fibrosis in the tracheotomy model group decrease? In the lumbar puncture needle tracheal intubation model group and the indwelling needle tracheal intubation model group, the heaviest model was 21 days; the distribution of each group was uneven, and the lung tissue around the trachea was heavy. 28 days after modeling, the levels of hydroxyproline in the lumbar puncture tracheal intubation model group and the tracheotomy model group were significantly higher than those in the blank group (P\u003c0.01?P\u003c0.001).

　　Conclusion: Combining multiple factors, is the lumbar puncture and tracheal intubation modeling method the best among the three modeling methods?