动物造模,姜黄素,2型糖尿病 z-bo 2020-09-10 363

　　Objective: To study the effect of curcumin on glucose transport and phosphatidylinositol kinase 3 (PI3K)/protein kinase B (Akt) signaling pathway in adipocytes of type 2 diabetes (T2DM) rats?

　　Methods: Randomly select 90 SD rats, normal group, model group (high-fat diet), low-dose curcumin group (50 mg/kg), medium-dose curcumin group (150 mg/kg), high-dose curcumin group (250 mg)/kg), randomly divided into Rogge. Ritalone group (1.35 mg/kg), 15 rats in each group? Take it immediately after taking it? 2 Take medicine on weekend Rats were weighed for 8 weekend doses, and blood was collected for 8 weekend doses and fasted for 12 hours. Fasting blood glucose (FBG) was tested whether fasting insulin (FINS) was tested? Calculate insulin resistance index (HOMA-IR), triglyceride (TG)? Low/high density lipoprotein cholesterol (LDC-C/HDL-C)? Total cholesterol (TC) expression? Positive glucose clamp test can measure glucose perfusion rate, fluorescence immunoassay can detect the displacement of adipocyte membrane glucose transporter 4 (GluT4), real-time fluorescent quantitative PCR (qRT-PCR) can detect GluT4 in adipocytes, Western blot can detect fat The inside and outside of the cell. GluT4? p-PI3K? Insulin receptor substrate 2 (Irs2)? Membrane p-Akt expression?

　　Result: Compared with the normal group, is the model group taken immediately? I gained weight after taking it for 2 weeks. Weight loss at the 8th week; compared with the model group, the curcumin treatment group and rosiglitazone group were less weight than the model group just 2 weeks after the administration (P\u003c0.05). FBG group? FINS? HOMA-IR? HDC-L? LDC-L? TC? TG increased and glucose infusion rate decreased in a dose-dependent manner; GluT4 mRNA increased, and GluT4α increased in the model group. Irs2? p-PI3K/PI3K? The expression of pAkt/Akt protein is significantly reduced; is the above difference statistically significant (P\u003c0.05)? Compared with the curcumin treatment group and the model group? ossigritazone group FBG? FINS? HOMA-IR? HDC-L? LDC-L? TC? TG level is significantly reduced, glucose infusion rate is significantly increased, GluT4 mRNA is reduced, GluT4? Irs2? p-PI3K/PI3K? The expression of pAkt/Akt protein increased significantly. The above difference is statistically significant (P\u003c0.05). Is the difference between the three curcumin treatment groups also statistically significant (P\u003c0.05)?

　　Conclusion: Curcumin can improve the insulin sensitivity of T2DM rats and may activate PI3K/Akt signaling. Therefore, this pathway promotes the GluT4 membrane transport of adipocytes and the absorption of glucose by adipocytes.