动物造模,葫芦巴碱 z-bo 2020-09-11 337

　　Objective: To study the protective effect of trigonelline on testicular damage in mice caused by heat stress?

　　Method: 120 male ICR mice were randomly divided into a normal control group, a model group and a triangular essence drug intervention group (25 mg/kg). , 50 mg/kg, 100 mg/kg), 24 mice per group? Except for the normal control group, mice in each group were given intragastric administration for 1 week, and the lower abdomen of each group was heated in a constant temperature water bath at 43°C. After 15 minutes of stress treatment, the dose of triamcinolone corresponding to the drug intervention group was forcibly fed for 14 days, and the normal control group and model group were given normal saline for 14 days. The material was collected after 7, 10 and 14 days. Do you want to calculate reproductive organ index, sperm count, HE staining to observe, compare and analyze the changes in testicular tissue structure?

　　Result: Compared with the normal control group, the upper limb reproductive organ index and sperm count of the model group were significantly reduced (P\u003c0.05), and the testes of the mice had histopathological changes. Are you damaged? Compared with the model group, each dose of the drug intervention group can increase the organ coefficient of the reproductive organs of the mice and the number of sperm on the clavicle (P\u003c0.05); the histopathology is whether the group can significantly improve the mice by drug treatment Testicular damage caused by heat stress?

　　Conclusion: Does Trigonelline significantly improve the damage of heat stress to the testis of mice and have specific protective effects?