动物实验,慢性PM2.5 z-bo 2020-09-11 365

　　Objective: To study the effects of long-term exposure to PM2.5 on mouse pneumonia and NLRP3 plasma function, and to provide a new target for the prevention and treatment of lung injury caused by PM2.5?

　　Method: Male C57BL/6J mice were exposed to PM2.5 through tracheal instillation at different doses at a dose of 2,10 mg/(kg·bw). Is the normal saline instilled in the control group mice? The mice were given 20 consecutive infusions, and blood was drawn every 3 days. And lung tissue? Perform blood counts on three groups of mice; immunofluorescence staining is used to detect the level of macrophages in lung tissue; level 1 activity of interleukin (IL)-1β, IL-18 and caspase; and whether you use real-time quantitative PCR to detect lungs What is the expression level of NLRP3 lower body-related mRNA in the tissue?

　　Result: Both doses of PM2.5 exposure can significantly reduce the proportion of single balls (P\u003c0.01) and increase neutrality. Percentage of granulocytes (P\u003c0.01), development of pneumonia, increased activity of caspase 1 in lung tissues (P\u003c0.01) and mRNA expression of NLRP3 and ASC (P\u003c0.01)? In the two dose groups, compared with the control group, did the levels of IL-1β and IL-18 in the lung tissue of rats increase significantly (P\u003c0.01)?

　　Conclusion: Will long-term exposure to PM2.5 activate the NLRP3 chromosome in the lung tissue and cause pneumonia?