动物实验,脑组织Bax表达 z-bo 2020-09-11 368

　　Objective: How to study the effect of psychologically dependent heroin on the development of embryonic rats and the expression of Bax in the brain?

　　Method: Wistar rats after pregnancy were randomly divided into control group and heroin administration group (divided into low, medium and high heroin dose) on the 7th day after pregnancy, with 16-32 mg and 64 mg/kg respectively. After taking heroin and continuously taking heroin for 9 days, the effect of heroin (a mentally dependent substance) on the morphological and structural development of embryonic rats was observed, and it was tested by enzyme immunoassay (ELISA). What is the expression level of Bax in brain tissue?

　　Results: Through embryo observation, the total number of viable embryos in the low, medium and high dose heroin groups were reduced by 27.27%, 37.12% and 48.48% respectively compared with the control group. The low dose heroin group was embryos. The occipital region is underdeveloped and the embryo's brain is protruding deformity; the occipital and parietal bones of the embryos in the medium-dose heroin group are underdeveloped, and the embryonic brain herniation is obvious; whether the embryonic brain herpes test in the high-dose heroin group shows the embryo's occipital bone and parietal bone And temporal bone hypoplasia, is it hypoembryonic brain herniation? The expression level of Bax in embryonic brain tissues of the medium and high dose groups increased by 11.41%, 47.06% and 83.74% respectively compared with the control group, the difference was statistically significant (P\u003c0.05, P). u003c0.01); the expression level of Bax in embryonic brain tissue is higher than that in the control group. The control group increased by 17.16%, 52.96% and 90.01% respectively, and the difference was statistically significant (P\u003c0.05, P\u003c0.01)?

　　Conclusion: The psychotropic drug heroin can significantly inhibit the morphological and structural development of embryonic rats. Is it possible to increase the dose of heroin? Its mechanism of action is related to the up-regulation of Bax expression in embryonic tissues and organs?