中洪博元,动物造模,重型胰腺炎 z-bo 2022-02-05 489

1. In addition to surgery, more commonly used and reliable establishment methods are mainly used: 5% sodium taurocholate, rats, rabbits, pigs, dogs and other animals have a variety of options for establishing acute severe pancreatitis model animals. Generally, methods such as pancreatic duct ligation, intrapancreatic duct and subcutaneous injection of sodium taurocholate are used.
(1) Establish a rat model of acute severe pancreatitis with 5% sodium taurocholate sodium. 1. Ge Zhong, Zhang Dongsheng, etc. Comparison of the therapeutic effects of panax notoginseng saponins and Shanning on acute severe pancreatitis in rats. 2002 Volume 12, Issue 14, "China Journal of Modern Medicine" ChinaJournalofModernMedicine opening and closing pages: 17-19, 22
 2. KristienVerhoeven, LutVanLaer. Karin Kirschhofer (Karin Kirschhofer). Wait. Humana mutation-ectrin is caused by autosomal dominant inheritance-hindlimb sympathetic neuropathy. atureGenetics, 1998: 19: 60? 62
 3. Li Qinghua[1] Xu Ping[2] Chen Lingquan. A modified rat model of severe acute pancreatitis. 2006, Volume 7, Issue 7, "Practical Clinical Medicine (Jiangxi)" Practical Clinical Medicine Start and End Page: 5% taurocholate passes through the bile duct and pancreatic duct near the open end of the duodenum, which can induce 1- 3,6 cases of severe acute pancreatitis were injected retrogradely with sodium (2), and 5% sodium taurocholate was injected retrogradely into the pancreaticobiliary duct to establish a rabbit SAP model. Salvia miltiorrhiza on rabbits with severe acute pancreatitis
Sha Jianping, Peter and Guan Ruhong The influence of the mitochondrial respiratory chain function of intestinal mucosal epithelial cells. 2003, Volume 26, Volume 3, "Journal of Chengdu University of Traditional Chinese Medicine", Chengdu University of Traditional Chinese Medicine, start and end: 1-3
(3) Inject 5% sodium taurocholate + own bile into the retrograde pancreatic duct to prepare a dog SAP animal model.
 1. Li Lei, Tang Yaoqing, Mao Enqiang, Qin Shuai, Chen Sheng, Zhang Mingjun. The mechanism of hemofiltration therapy for severe acute pancreatitis. 2004, Volume 12, Issue 12, "World Chinese Journal of Digestion", opening and closing pages: 2822-2825
 2. Zhu Qi, Xia Lu, Shen Jun, Yuan Yaozong, Xu Jiayu. The effect of eon glucoside on the sphincter of Oddi in dogs with acute severe pancreatitis. 2004, Volume 12, Issue 2, "Chinese Journal of Integrated Traditional Chinese and Western Medicine on Digestion" Chinese Journal of Integrated Traditional Chinese and Western Medicine on Digestion Start and end pages: 77-79
(4) Sodium taurocholate can induce a pig model of severe acute pancreatitis.
Tu Weifeng, Zhu Weiming, He Jing, Li Jieshou. Pathological features of lung injury after acute severe pancreatitis in swine induced by sodium taurocholate. 2003, Volume 19, Issue 10, opening and closing pages: 1079-1081
 2. Establish a mouse model of severe acute pancreatitis by combining apiline and lipopolysaccharide
 Kinhae, Li Zhicheng, the combination of ceruloplasmin and lipopolysaccharide can cause severe acute pancreatitis in mice. 2002, Volume 24, Issue 4, "ActaAcademiaeMedicinae Sinicae", opening and closing pages: 393-396, F003
Combined use of small molecule cellulose and intraperitoneal administration of lipopolysaccharide in mice; measurement of blood amylase and pancreatic water content. Pathology of pancreas and extra-pancreatic organs. Results The combination of cervicin and lipopolysaccharide drugs can increase blood amylase and pancreatic water content, accompanied by pancreatic interstitial edema, substantial hemorrhage and necrosis, inflammatory cell infiltration, and different degrees of damage to the stomach, ileum, spleen and lungs; In the penicillin group, there was no obvious pancreas, hemorrhage or necrosis, normal organs outside the pancreas. Discussion The mouse model of severe acute pancreatitis caused by the combination of cerebellin and lipopolysaccharide shows the pathological changes of human severe acute pancreatitis. It is non-invasive, easy to operate, modularized, fast and stable, and reproducible. 3. Use sodium deoxycholate, bovine bile salt, chymotrypsin, bile and blood to inject Wakayama's method into the main pancreatic duct of mongrel dogs to establish an experimental animal model of severe acute pancreatitis.
The rapid development and screening of experimental animal models of severe acute pancreatitis by Xu Lin, Darong. 1996, Volume 24, Issue 1, "Journal of PLA Medical College", the beginning and ending page numbers of "Journal of Clinical Medicine": 21-24