动物实验,氧化苦参碱,肝癌 z-bo 2020-09-11 404

　　Objective: How to observe the combined inhibition of oxymatrine (OMT) and all-transetinoic acid (ATRA) on the formation of rat liver cancer induced by diethylnitrosamine (DEN) and explore its mechanism of action?

　　Method: Use a rat model of DEN-induced liver cancer and establish the model for 18 weeks. Wistar rats were randomly divided into blank group, model group and intervention group. The rats were sacrificed 6-12 and 18 weeks after the cancer was induced. Observe the pathological changes of rat liver tissue by staining, detect the relevant indicators of rat liver function, and detect rat IL-6 content by ELISA, rat let-7a, and RT-qPCR. F-κBp65? Detect the changes of IL-6 and STAT3 mRNA expression. Do you use Western blotting to detect protein changes in STAT3? Is it rat p-STAT3?

　　Results: The pathological observation of HE staining showed that the number of liver cancer nodules in the intervention group was significantly less than that in the model group, and liver cell necrosis was less than that in the model group. -6 and liver function index ALT? GGT? AST? Compared with the model group, the ALP level of the intervention group was significantly reduced (P\u003c0.05); T-qPCR test results: let-7amRNANF-κB-p65, IL-6 and liver tissue. The expression level of STAT3 mRNA decreased significantly, while the expression level of STAT3 mRNA increased significantly (P\u003c0.05). In the 18th week of the experiment, the Western blot test results showed that the expression of STAT3 and p-STAT3 protein in the intervention group was significantly lower than that of the model. Group (P\u003c0.05)?

　　Conclusion: Can oxymatrine combined with all-trans retinoic acid significantly inhibit the formation of DEN-induced liver cancer in rats and delay tumor growth to a certain extent?