动物实验,TRPV1 z-bo 2020-09-11 379

　　Objective: To observe the relationship between TRPV1 and P2X3 in rat peripheral blood, in order to partially clarify the sensory regulation mechanism of peripheral pain?

　　Method: Did you randomly divide male SD rats into a blank control group? TRPV1 agonist group? P2X3 agonist group? TRPV1 agonist + P2X3 agonist group? TPRV1 agonist + P2X3 inhibitor group? P2X3 agonist + TRPV1 inhibitor group? TRPV1 or P2X3 agonists and/or inhibitors were injected subcutaneously into the soles of the feet, and the rats in each group were observed to contract within 20 minutes. Number of legs? Leg lift/foot licking time; use immunofluorescence to observe the expression and co-expression of TRPV1 and P2X3 positive regions at L4DRG level; use immunoprecipitation to observe TRPV1 and P2X3 at L4DRG level. Have you observed this relationship?

　　Result: P2X3 inhibitors that stimulate P2X3 cannot improve the painful behavior caused by TRPV1 agonists. P2X3 inhibitors can reduce the painful behaviors caused by TRPV1 agonists. TRPV1 agonists can increase painful behaviors caused by P2X3 agonists. TRPV1 inhibitors will not reduce the painful behaviors caused by P2X3 agonists. P2X3 agonists can increase the expression of TRPV1 positive regions at the L4DRG level, and TRPV1 agonists can increase the expression of P2X3 positive regions at the L4DRG level; TRPV1 and P2X3 are co-expressed at the DRG level, do you sink together?

　　Conclusion: Is there a specific interaction between TRPV1 and P2X3 at the level of peripheral neurons? Can the two promote each other's expression? If one of them is suppressed, will the other function be reduced accordingly?