动物实验,流感病毒 z-bo 2020-09-11 353

　　Objective: How to observe the effect of compound Chinese medicine on lung injury in mice infected with influenza A/PR/8/H1N1 virus?

　　Method: Divide 90 female BALB/c mice randomly into an empty control group? The virus control group was divided into 6 groups, each with 15 animals, including the amatazidine hydrochloride group and the Chinese medicine high-dose, medium-dose and low-dose groups. In the traditional Chinese medicine group, the mice were injected intranasally after intragastric administration for 2 consecutive days to establish a mouse model of influenza virus, and the blank control group was given a nasal drip. Four hours after the virus infection, each group received different doses of drugs or normal saline, and then forced oral administration for 5 days. On the 5th day of infection, the lung index of each group of BALB/c mice was calculated, and the thrombosis of lung tissue homogenate was measured. Have you observed the pathology of lung tissue?

　　Results: Compared with the virus control group, the lung index of the amatazidine hydrochloride group and the middle-dose combined Chinese medicine group was significantly reduced (P\u003c0.01), while the lung index of the low-dose combined Chinese medicine group and the high-dose Chinese medicine preparation group . The index has dropped significantly (P\u003c0.05). The coagulation titer of lung tissue of mice in each drug group was significantly reduced (P\u003c0.01). The lung tissues of mice in each drug group had different degrees of pathological morphology.

　　Conclusion: The compound Chinese herbal formula used in this study significantly reduced the average lung index and average coagulation titer of mice infected with the A/PR/8/H1N1 influenza virus and caused lung injury.