动物实验,骨髓干细胞移植 z-bo 2020-09-11 356

　　Recently, researchers said that the combination of two clinically approved drugs (Viagra and Prixafor) in the "Stem Cell Report" can quickly and effectively regulate the entry of blood stem cells in the bone marrow of mice into the blood. Said. This strategy is as effective as current standard protocols for hematopoietic stem cell recruitment.

　　"These two drugs have been approved by the U.S. Food and Drug Administration (FDA) and can be tested relatively quickly by human volunteers." Stem cell biologist and corresponding author at the University of California, Santa Cruz. "If clinical studies prove to be safe and effective, doctors can consider these findings."

　　Hematopoietic stem cell transplantation can make abnormal hematopoietic stem cells healthy. It is a method to treat various blood and immune diseases by replacing cells. In this process, hematopoietic stem cells in the bone marrow are mobilized into the blood, and then these cells are collected and transplanted. It can be transplanted to the same person or recipient. However, the main obstacles to hematopoietic stem cell transplantation are the limited supply of donor cells and the lack of effective means to obtain them. Currently, the standard protocol for mobilizing hematopoietic stem and progenitor cells includes injection of granulocyte colony stimulator (G-CSF) for several days. Although G-CSF is effective in most cases, it is expensive, causes side effects such as nausea, fatigue and bone pain, and is generally ineffective for cancer patients receiving chemotherapy. Another drug that can activate hematopoietic stem cells is Prixafor, but it is not very effective as a single drug. Forsberg said: "A better harvest plan will greatly increase the success rate of current indications and open treatment hematopoietic cell therapy." Recent experiments have shown that increased vascular permeability helps mobilize hematopoietic stem cells from the bone marrow to the blood. in. Based on this, the Forsberg team tested whether Viagra can be a viable option for hematopoietic stem cell transplantation.

　　Researchers found that Viagra itself is ineffective. This shows that people currently taking Viagra for other reasons do not have to worry about accidentally mobilizing hematopoietic stem cells. Currently, the number of hematopoietic cells in mice using only Prisafox is almost three times that of control mice. However, in combination therapy, it is best to take Viagra once and inject Prixafor into mice, and within 2 hours, there are approximately 2500 hematopoietic stem cells in the blood. This is 7.5 times of controlling the mouse. After oral administration of Viagra for 3 days and a single injection of Prixafor, the number of hematopoietic stem cells increased by about 2,800, which was 8.4 times that of control mice. In addition, the effectiveness of this method is comparable to 4-day G-CSF treatment. However, the researchers said that further studies on humans are needed to test the safety and effectiveness of this method.