动物实验,KK鼠料 z-bo 2020-09-11 416

　　Objective: How to quickly model KK mouse feed and study its effect on related indicators of KK/Upj-Ay/J mice?

　　Method: Divide into 4 groups, and use 40 female and male KK/UpjAy/J mice for experiment. , Normal feeding group (10 males, 10 females), KK rat feeding group (10 males and 10 females), normal fasting blood glucose group (10 males and females), KK young rats fasting blood glucose group ( Female type) (10 males each) feed from 3 to 23 weeks until the end of the experiment. Measure body weight and fasting blood glucose every week. After the experiment, serum was taken as biochemical indicators, and the pancreas, kidneys and liver were fixed for HE and special staining (pancreas was used for insulin immunohistochemical staining, liver was used for PAS staining, and PASM staining was used for PASM staining). kidney)

　　Result: The diet of KK rats significantly promoted the weight gain of KK/Upj-Ay/J mice and increased the fasting blood glucose level of KK/Upj-Ay/J mice. Increase the content of alanine aminotransferase in the serum of J mice (P\u003c0.05)? Feeding KK rats can significantly increase the serum creatinine content of female KK/Upj-Ay/J mice (P\u003c0.05). 0.05), significantly increased the serum cholesterol content of male KK/Upj-Ay/J mice (P\u003c0.05), but significantly increased the serum cholesterol content of female KK/Upj-Ay/J mice Does it affect? At the end of the feeding experiment, the pathological changes of the pancreas of female KK/Upj-Ay/J mice are obvious, but does it have a significant effect on the pathological changes of the male KK/Upj-Ay/J mice? Is it related to the physiological characteristics of male KK/Upj-Ay/J mice? Can the diet of KK rats significantly promote fatty liver in KK/Upj-Ay/J mice? At the end of the feeding experiment, what are the main manifestations of renal pathological changes in the normal diet group compared with the C57BL/6J group? Did renal tubulin cast, interstitial nephritis, perivascular inflammation and mesangial matrix increase in the KK rat feed group? Is the degree of kidney disease more obvious in the KK feeding group? The results of kidney PASM staining showed that compared with the control group, there were two other groups: Is there no dark brown substance in the glomerular basement membrane, indicating that the glomerular basement membrane structure is damaged?

　　Conclusion: The diet of KK rats promotes the damage of diabetic organs in KK/Upj-Ay/J mice, and increases fasting blood sugar and body weight. Do you want to reduce modeling time and improve modeling uniformity?