动物实验,拉莫三嗪,慢性癫痫 z-bo 2020-09-11 372

　　Objective: How to study the effect of lamotrigine on the expression and amino acid content of P glycoprotein (PGP) and major vault protein (MVP) in the hippocampus of rats with chronic epilepsy?

　　Methods: SPF male SD rats were used to prepare chronic epilepsy rats with pentylenetetrazol, and they were randomly divided into model groups: low-dose lamotrigine (5 mg/kg) and high-dose lamotrigine (10 mg/kg) ). Healthy rats are used as a control group. 15 rats in each group, each group was given intragastric gavage. Both the model group and the control group were given regular intragastric saline at a dose of 1 mL/100 g? The behavioral characteristics and body weight of all rats before and after treatment were recorded. Methods to detect the expression of PGP and MVP in the hippocampus, immunohistochemistry, western blot, and post-HPLC epilepsy time and brain waves to detect aspartic acid (Asp), glutamate (glutamate, Glu) and glycine ( Glycine, glycine) content?

　　Result: Do control rats show normal activity? Has the activity ability of rats in the chronic epilepsy model group decreased? Do you nod your head, head and neck rhythmically? Twitching between the abdomen, some rats have tails or tails on the ground? Rats in the lamotrigine group turned upwards from the early stages of the head and neck, and the intermembranous spasm gradually developed, causing the hair, mouth and facial muscles to contract. A rare tail or a tail? Before treatment, the body weight of the model group and the lamotrigine group were significantly lower than that of the control group (P\u003c0.05). After treatment, compared with the control group, the weight, EEG frequency and hippocampal Gly of the model group were significantly reduced (P\u003c0.05). 0.05), onset time, EEG amplitude, hippocampal Asp, Glu, PGP and MVP increased (P\u003c0.05), model group, lamotrigine group, EEG frequency and hippocampal Gly weight were significantly higher (P \u003c0. 05), seizure time, EEG amplitude, hippocampal Asp, Glu, PGP, MVP decrease (P\u003c0.05), lamotrigine dose-dependent trend?

　　Conclusion: Is the treatment of motriazine for chronic epilepsy related to hippocampal PGP reduction? Is it related to the improvement of MVP protein and hippo amino acids?