动物实验,人脐带间充质干细胞移 z-bo 2020-09-11 413

　　Objective: To explore the transplantation of human umbilical cord mesenchymal stem cells (hUCMSC) and the mechanism of repairing ovarian function in ovarian failure (POF) rats?

　　Method: 30 SPF SD female rats were randomly selected as controls. Another 20 rats were established in the POF model, and the model rats were divided into model group (10) and hUCMSC transplantation group (10). Observe the changes in the ultrastructure of follicles and ovaries in each group, and detect serum by ELISA. E2? FSH? AMH? OS? At the level of 8-OHdG, is immunohistochemistry and western blotting used to detect changes in rat SOD1 and UCP2 proteins?

　　Result: HE staining showed an increase in the number of follicles in the model and the control group. The number of growing follicles decreased (all P\u003c0.05). Compared with the model group, the number of obturator holes in the hUMSCs transplantation group decreased, and the number of follicle growth increased (all P\u003c0.05). ELISA results showed that the serum LH, FSH levels, ROS and 8-OHdG production in the hUCMSCs transplantation group were the model group (all are P\u003c0). Compared with the control group is significantly lower than 05)? Compared with the two groups, the E2 level of hUCMSC transplantation group was significantly increased (P\u003c0.05). The immunohistochemical results show that the model group immunohistochemical UCP-2 compared with the control group? hUCMSC transplantation group UCP-2? Increased the number of SOD1 positive cells? Compared with the model group, the number of SOD1 positive cells decreased; Western blot results showed that the ovarian tissue of the model group was SOD1 positive. The expression of UCP-2 protein was significantly higher than that of the control group (P\u003c0.05). After hUCMSC transplantation, SOD1? Is the UCP-2 protein level significantly lower than the model group (P\u003c0.05)?

　　Conclusion: Does hUMSC transplantation play a role in reducing the expression of SOD1 and UCP-2, thereby reducing oxidative stress and ultimately repairing ovarian function?