动物实验,糖尿病肾病 z-bo 2020-09-11 374

　　Objective: How to study the effect of quetiacyclohexanone on diabetic nephropathy in rats and the effect on the expression of angiotensin II (Ang II) and vascular endothelial growth factor (VEGF) in the kidney?

　　Method: Select 40 rats for 8 consecutive weeks, and randomly select 10 healthy SD rats as the normal group, and the remaining 30 are used to establish a rat model of diabetic nephropathy. After successful modeling, the two groups of diabetic nephropathy rats were randomly divided into 10 model groups and 20 treatment groups. Five rats in the drug group were injected with the same dose and gavage with 5-10-15-30 mg/kg quinsulfone and cyclohexanone. The rats in the control group and the model group were given normal saline. oral. Group: Normal group rat urine albumin excretion rate (Uaer), glomerular filtration rate (GFR), fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), insulin resistance index (HOMA-IR) 24 hours urine Microalbumin (24hU-mAlb), β2 microglobulin (β2-MG), blood urea nitrogen (BUN) level) mRNA, used to detect oncoprotein 1 (MCP-1)? Lectin-like oxidized low-density lipoprotein receptor (LOX-1) mRNA and AngII? The expression level of VEGF, and the comparison between the groups? The resulting dose is 15 mg/kg Uaer? Is the rat GFR level normal? Due to the GFR level, 15 mg/kg is considered an appropriate dose. The following study will be conducted at a dose of 15 mg/kg. FBG in the model group and the administration group? HbA1c? HOMA-IR? 24hU-mAlb? β2-MG? The BUN level is higher than the normal group (P\u003c0.05); the model group? MCP-1 mRNA? LOX-1 mRNA? What is the model effect of Ang II in rat kidney tissue? VEGF expression level? (U003c0.05); Is FBG in the administration group? HbA1c? HOMA-IR? 24hU-mAlb? β2-MG? The BUN level was lower than the model group (P\u003c0.05). LOX-1 mRNA? AngⅡ? The expression level of VEGF is lower than the model group, and there is a statistical difference (P\u003c0.05)?

　　Conclusion: Is auracyclohexanone an AngII in the kidney tissue of rats with diabetic nephropathy? May inhibit the expression of VEGF, improve renal function and disease in rats, and ultimately play a role in renal protection