动物实验,骆驼刺提取物 z-bo 2020-09-11 346

　　Objective: How to actively study the effect of camel thorn extract pretreatment on the viability of rats in a dry environment?

　　Method: 50 SPF male SD rats were randomly divided into 5 groups: blank group (NC group) and solvent group (SC group)? Low-dose camel thorn group (AL group)? Medium dose camel thorn group (AM group)? High-dose camel thorn group (AH group), 10 per group? The SC group was given a 0.5% sodium carboxymethyl cellulose solution forcibly orally, and the high, medium and low doses of camel thorn extract groups were given 1 g/kg, 0.33 g/kg, 0.10 g/kg, and each group was given orally every day for 7 consecutive days Rat once. Do you put it in a laboratory in a dry, high-temperature environment with a temperature of (41±0.5)°C and a relative humidity of (10±1)%? Do you want to record the core body temperature, body weight and time of death of the rats separately, 0-50-100-150 minutes?

　　Result: During the continuous administration period, there was no significant difference in the daily weight change of rats in each group (P\→0.05). After being exposed to dry heat for 50-100-150 minutes, the core body temperature of the AM AH group is the NC SC group (P0.05)? What is the survival time of rats in the AM group in a dry environment [(242.15±12.72) minutes]? Does the AH group [(244.90±12.68) minutes] compare with the NC group [(225.90±10.32) minutes]? SC group [(228.22)±7.59)min]? The AL group [(227.90±12.25) min] was significantly prolonged (P\u003c0.05). Through LogRank test, the survival rate of NC was NC. SC? Morning than AL group? Is the AH group significantly higher (P\u003c0.05)?

　　Conclusion: The pretreatment of camel thorn extract can slow down the increase in core temperature and improve the heat tolerance of rats in arid environments, thereby increasing the survival rate of rats in desert and arid environments. The first study showed that there is sex. Has this mechanism been further studied?