动物实验,参枝苓口服液,App/Ps1双转基因 z-bo 2020-09-11 497

　　OBJECTIVE: By observing the changes of myelin sheath and synapse microstructure in early hippocampal CA1 area of App/Ps1 double transgenic mice and the special staining of myelin sheath in the early stage of App/Ps1 double transgenic mice 3 months ago, early neuroprotection in AD mice. How to determine the initial neuroprotective effect of Shenzhiling Oral Liquid on the App/Ps1 transgenic mouse model formed by mating PrP-hAppk595N/M596L single transgenic dementia model mice with PrP-hPs1dE9 single transgenic dementia model mice? Is a common disease model of Zheimer's disease? Are the 3-month-old App/Ps1 double transgenic mice randomly divided into model groups? Donepezil group [0.92mg /(kg·D)], Shenshiling high-dose group [50g /(kg·D)]? Shenzhiling middle dose group [25g /(kg·D)]? In the low-dose group Shenzhiling [12.5g/(kg·D)], 9 mice in each group, in the same month as the control group, 9 C57BL/6J mice with the same background? Mandatory feeding for 3 consecutive months. After the experiment, an electron microscope was used to photograph the tissues of the CA1 area of the mouse hippocampus to observe the myelin sheath and synapse-related ultrastructure in this area. Have you stained the myelin and analyzed it with the neuromyelin rapid blue staining method?

　　Results: When observed with an electron microscope, the number of synapses in the CA1 region of the hippocampus of the model group decreased (P\u003c0.01), and the neuron structure showed degenerative changes compared with the control group. Do you? The condition and structure of the thin myelin layer has been severely collapsed. Compared with the model group, does the number of synapses in the CA1 region of the hippocampus of mice in each drug intervention group increase (P\u003c0.01), and are the morphology and structure of neurons and oligodendrocytes more complete? Is the chromatin uniform and the myelin structure significantly improved? Have you found that compared with the control group, the special staining method can significantly reduce the myelin fibers in the model group? Did the stain become lighter? The distribution of myelin fibers in each intervention group increased to varying degrees, and did the stain darken?

　　Conclusion: Shenzhiling oral liquid can provide early AD neuroprotection by increasing the synaptic count in the hippocampal CA1 area of App/Ps1 double transgenic mice and improving the structure and morphology of mierin, oligodendrocytes and neurons. Is it possible to prove this?