动物实验,低氧训练 z-bo 2020-09-14 347

　　Objective: We conducted hypoxic training on obese mice, observed the changes of grelin and hypothalamic GHSR levels in the stomach tissue, and studied whether hypoxic training affects the grelin GHSR pathway to improve obesity.

　　Method: Male C57BL/6J mice were randomly divided into a normal control group (C, n = 8) and a high-fat diet group (H, n = 52). After the obesity model was established in group H, they were randomly divided into obesity control group (HC), obesity normoxic exercise group (HE), obesity hypoxic exposure group (HH), obesity hypoxic exercise group (HHE), and each exercise group had moderate running Machine training, intermittent hypoxic exposure (oxygen content of 11-2%) in each hypoxic group, weekly weight and food intake for 4 weeks, serum TC, TG, GLU and total grelin levels were detected by RT-PCR method , And detect the mRNA expression levels of hypothalamic GHSR and gastric grelin, WB detects the GHSR and NPY protein content of chest, grelin, goats, and HIF_2α protein content in stomach tissue.

　　Result: (1) After 4 weeks of intervention, the weight of HE, HH and HHE groups was significantly lower than that of HC group. In the early stage of hypoxic intervention, the food intake of the HH and HHE groups decreased, but as the intervention time increased, the food intake of the obesity group gradually approached. (2) The blood index of HC group was significantly higher than that of C group. The TC level of HH group was significantly lower than that of HC group. The GLU level of HE, HH and HHE group was significantly lower than that of HC group. Compared with HH and HHE HC groups, the total serum grelin level of HC group was significantly lower (3) Hypothalamus GHSRMRNA and gastric grelin mRNA levels of HC group were significantly lower than those of C group. it is. 4) Hypothalamus GHSR protein levels in HE, HH and HHE groups were significantly higher than HC group, and NPY protein levels in HE and HHE groups were significantly higher than HC group (5). The levels of gastric Grelin protein in HE, HH and HHE groups were significantly higher than those in HC groups; goat protein levels in HE and HHE groups were significantly higher than those in HC groups, and HIF2α in HH and HHE groups were significantly higher than those in HC groups. The protein level was significantly higher than the HC group.

　　Conclusion: Hypoxic training can affect the glucose and lipid metabolism and weight loss of obese mice by regulating the grelin GHSR signaling pathway.