动物实验,糖尿病神经痛 z-bo 2020-09-14 374

　　Objective: To explore the partial analgesic mechanism of electroacupuncture in the treatment of diabetic neuropathy, and the effect of electroacupuncture on p65 protein in the posterior horn of spinal cord in rats with diabetic neuropathy (diabetic neuropathy pan, DNP).

　　Method: 30 SD rats were divided into normal group and model by completely random grouping method. These rats were fed a normal diet and a high-fat, high-sugar diet for 5 weeks. Five weeks later, a model rat was given, after intraperitoneal injection of streptozotocin (STZ), the rats that successfully simulated the model of diabetic neuropathy were randomly divided into model group (DNP group) and model + electroacupuncture Group (DNP group). DNP + EAGrup) and dual-acupuncture point selection "Zusanli, Kunlun Point" side, frequency is 2Hz, 1mA intervention intensity is 15 minutes, 2mA intervention is 15 minutes, from the 7th week and the 1st week, the intervention is performed once a day, a total of 7 times. Observe the changes of blood glucose level, mechanical pain threshold and thermal pain threshold of each group of rats. Hematoxylin-eosin (HE) staining was used to observe the morphological changes of rat sciatic nerve, and the posterior horn of rat spinal cord was detected by western blotting. The expression level of F-κBp65 protein.

　　Result: After high-fat and high-sugar feeding for 5 weeks, rats were given an intraperitoneal injection of STZ for 2 weeks. Blood sugar rises, mechanical and fever thresholds decrease. This indicates that the DNP rat model has been successfully established, and electroacupuncture can up-regulate DNP rats. The mechanical pain threshold and fever pain threshold of DNP have no obvious intervention effect on blood sugar. The results of HE staining showed that the nerve fibers of DNP rats were arranged randomly, axons were swollen, myelin density was uneven, follicular degeneration, and electroacupuncture intervention. There is no obvious effect on the shape. Western blotting results showed that the expression of NF-κBp65 protein in the spinal dorsal horn of DNP rats was significantly increased, and electroacupuncture could down-regulate the expression of NF-κBp65 protein in the spinal dorsal horn of DNP rats.

　　Conclusion: Low-frequency electroacupuncture has a good analgesic effect in DNP model rats, which may be related to the inhibition of the expression of NF-κBp65 protein in the posterior horn of the spinal cord of diabetic DNP rats.