动物实验,高脂血症,肝脂肪变性,肠道菌群 z-bo 2020-09-14 316

　　Objective 　 To investigate the regulation effect of ferulic acid (ferulicacid) on hepatic steatosis and intestinal flora in hyperlipidemia mice.

　　Methods Twenty-four 6-week-old male ApoE-/- mice were randomly divided into 4 groups (n=6), namely control group, model group, ferulic acid group [40mg/(kg.d)], simvastatin group [5mg/(kg.d)], another 6 C57BL/6 mice of the same age were taken as the blank group. After 12 weeks of high-fat feeding, the mice were treated with administration for another 12 weeks. The feces of the mice were collected for intestinal flora detection, blood lipid levels were detected, and liver sections were made to observe pathological changes.

　　Results Compared with the control group, the body weight, serum total cholesterol (total cholesterol, TC), triglyceride (TG), low-density lipoprotein (low-density lipoprotein (Pol) and LDL) of the model group increased significantly. 0.05), the liver section showed that the mouse liver cytoplasm was filled with lipid droplets, and most of the liver cells showed fatty degeneration. The number of bacteria in the model group Firmicutes and Erysipelotrichaceae increased, Bacteroides Compared with the model group, the body weight, serum TC, TG, LDL-C of the mice in the ferulic acid group significantly reduced liver steatosis, and the phylum and Firmicutes were significantly reduced. The number of Veillonococcus bacteria decreased, and the number of Bacteroides, Rumenbacteria, and Odoribacter increased.