动物实验,脑缺血,莪术二酮 z-bo 2020-09-14 374

　　Objective 　 To investigate the effects of curcuma dione on cognitive function, neurological function and mechanism of cerebral ischemia-reperfusion injury in mice.

　　Methods A mouse model of cerebral ischemia-reperfusion injury was established by the suture method. The low-molecular-weight heparin sodium group, the low- and high-dose turmeric dione group and the model were given 1 mg/(kg.d) and 20 mg/( respectively). kg.d), 60mg/(kg.d), observe the Morris water maze test, behavioral score, cerebral infarction rate, brain tissue water content in each group of mice, and determine serum 6-keto-PGF1α and 6-keto-PGF1α/ The ratio of TXB2, the expression level of cAMP and p-CREB in brain homogenate.

　　Results Compared with the sham operation group, mice in the model group had significantly longer escape latency, significantly reduced the number of platform crossings (P<0.01), behavioral score, cerebral infarction rate, brain tissue water content, and serum TXB2 content significantly increased (P <0.01), serum 6-keto-PGF1α, 6-keto-PGF1α/TXB2 ratio, brain homogenate cAMP, p-CREB were significantly reduced (P<0.01), compared with the model group, curcuma In the drug group, the escape latency was significantly shortened (P<0.05), the number of platform crossings was significantly increased (P<0.05), the behavioral score, cerebral infarction rate and brain tissue water content were significantly reduced (P<0.05), serum TXB2 content was significantly reduced (P<0.01), serum 6-keto-PGF1α, 6-keto-PGF1α/TXB2 ratio, brain homogenate cAMP and p-CREB were significantly increased (P<0.05), and showed Dose dependent.

　　Conclusion　 Curcuma dione has a good protective effect on the cognitive and neurological functions of mice with cerebral ischemia-reperfusion injury, and its mechanism may be related to the improvement of microcirculation disorders and activation of the cAMP/CREB/BDNF signaling pathway.