动物实验,淋巴细胞,狼疮小鼠 z-bo 2020-09-14 463

　　Objective To observe the effect of hydroxychloroquine on the distribution of lymphocytes in peripheral immune organs (spleen) of mice with systemic lupus erythematosus (TC mice).

　　Methods 30-week-old female TC mice were randomly allocated. The experimental group was given hydroxychloroquine treatment by gavage [10 mg / (kg·d)], and the control group was given normal saline treatment by gavage [10 mg / (kg·d)]. d)], a total of 5 weeks of treatment. Enzyme-linked immunosorbent assay (ELISA) was used to detect mouse peripheral blood anti-dsDNA antibody titer, hematoxylin-eosin (HE) staining method was used to detect renal tissue pathological changes, and flow cytometry was used to detect mouse spleen lymphocyte ratio. .

　　Results Compared with the control group, the renal histopathological score of the experimental group was significantly lower than that of the control group (P<0.05); the ratio of splenic B lymphocytes and germinal center B lymphocytes in the experimental group was significantly lower than that of the control group (P<0.05). 05); the proportion of spleen regulatory T lymphocytes (Treg) in the experimental group was significantly higher than that in the control group (P<0.05); there was no significant difference in the changes in the levels of anti-dsDNA antibodies and the proportion of Th17 lymphocytes in the two groups of mice (P<0.05);>0. 05).

　　Conclusion Hydroxychloroquine has a regulatory effect on the immune response of lymphocytes in peripheral immune organs of lupus mice and can alleviate the pathological damage of kidney tissue in TC mice.