动物实验,抑郁及焦虑 z-bo 2020-09-14 397

　　Objective: To observe and compare the depression and anxiety-like behaviors of experimental mice of different ages.

　　Method: There were 27 male C57BL/6 mice in the young group (3 months, n=9), middle-aged group (10 months, n=9) and old group (18 months, n=). I separated. 9) Carry out tail suspension test, forced swimming test, elevated plus maze test, open field test and sugar water preference test to observe depression and anxiety-like behaviors in mice, and use the LC-MS/MS method. C57BL/6 mice were used to detect serotonin content.

　　Result: The tail suspension time of the middle-aged group of mice was longer than that of the old group of mice (P\u003c0.05). In the young, middle, and old groups, the immobility time of forced swimming was significantly reduced, while the immobility time of swimming in the young group was in the middle-aged and older mice (P\u003c0.001). Significantly longer than the group of mice (P\u003c0.001), the swimming time of the middle-aged mice was significantly longer than that of the old group mice (P\u003c0.01); the swimming time of the middle-aged mice was 12 hours per hour The sugar preference rate was higher than that of the elderly group (P\u003c0.05), and the sugar preference rate of the young 12, 36, 48 and 60-hour groups was higher than that of the elderly group (P\u003c0.05). (U003c0.05), compared with the middle and young group, the open arm of the old mice is longer (P\u003c0.05), and the young group has more probes than the middle-aged group (P\u003c0. 05) (u003c0.01), younger groups are in the public domain than older groups. The total travel distance of the middle-aged group was longer (P\u003c0.05). The serum 5-HT content of mice in the old, middle-aged and young groups tended to decrease, while the serum 5-HT of the young group showed a downward trend. Significantly lower than the middle-aged group (P\u003c0.05). ) And the elderly group (P\u003c0.01).

　　Conclusion: In general, under the same stimulation, young mice are more likely to show depression and anxiety, and have lower serum 5-HT levels.