动物实验,星形胶质细胞 z-bo 2020-09-14 332

　　Objective To establish a rat astrocyte aging model to simulate the aging of astrocytes in aged rats, and to explore the effect of conditioned medium of aging astrocytes on the proliferation of neural stem cells.

　　Methods Trypsin digestion was used to isolate neonatal rat cortical astrocytes and 15-day fetal rat brain neural stem cells, 200 μmol/L H2O2 induced astrocytes for 4 h to establish an aging model; collected and cultured for 3 days and 7 d The supernatant of senescent cells was used as a conditioned medium, and the neural stem cell proliferation medium was added at a ratio of 1:3 or 1:2, and the effect on the proliferation function was observed by the count of neural stem cells and neurospheres. Conditioned medium served as a control.

　　Results 3 days of normal conditioned medium had no effect on the short-term proliferation of neural stem cells, but had an inhibitory effect on long-term proliferation; 3 days of aging conditioned medium inhibited the proliferation of neural stem cells; 7 days of normal conditioned medium promoted the short-term proliferation of neural stem cells, which had a significant effect on long-term proliferation. Inhibition; 7-day aging conditioned medium inhibited the proliferation of neural stem cells, and the inhibitory effect was greater than that of normal conditioned medium.

　　Conclusion H2O2 induces senescent astrocytes to inhibit the proliferation of neural stem cells, and the inhibitory effect on long-term proliferation is greater than that of normal astrocytes.